Proteinase K

Proteinase K

Protease K is a serine protease with efficient enzymatic activity and broad substrate specificity, preferentially breaking down ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and has two Ca2+ binding sites around the active center to increase its stability, allowing it to maintain high enzymatic activity under a wider range of conditions.

Technical Features

Attributes                                                    

Application

1.     Genetic diagnostic kits;

2.      RNA and DNA extraction kits;

3.     Extraction of non-protein components from tissues, degradation of protein-like impurities, such as DNA vaccine and heparin preparation;

4.      Preparation of chromosomal DNA for pulse electrophoresis;

5.      Protein blotting;

6.      Development and mass production of enzymatic glycated albumin reagents for IVD reagents.

Precaution

Wear protective gloves and goggles and keep well ventilated. The product may cause allergic skin reactions.