BunnyQuant DsDNA Hs Assay Kit

Product Description

BunnyQuant dsDNA HS Assay Kit is a fluorescent quantification kit for the specific detection of double-stranded DNA (dsDNA). The product is highly selective for dsDNA with good linearity in the 0.2-100 ng range. It can accurately detect dsDNA from various sources including genomic DNA, cell-free DNA, PCR amplification products, sequencing libraries, etc. The detection process is easy, safe and efficient, which is ideal for the quantification of NGS DNA samples.

Product Advantages

1. Easy Operation: Mix the sample with the fluorescent reagent and after 2 minutes, use Qubit fluorometer or Elisa reader to test.

2. High Sensitivity: The detection limit is lower to 200 pg.

3. Good Tolerance: Tolerate to higher concentrations of salt, urea, ethanol, chloroform, detergents, proteins or agarose and other conventional contaminants.

4. Broad Compatibility: Compatible with DNA quantification from multiple sample sources (plasma, blood, FFPE, tissue, alveolar lavage, swabs, etc.).

Experimental Data

Figure 1-2. High agreement of quantification results with reference standard and competitive product.

Using the BunnyQuant dsDNA HS Assay Kit and the ThermoFisher relevant kit, 10 dsDNA standards (Lambda DNA) at known concentrations were quantified using Qubit fluorometer and Elisa reader, respectively, and the results of the assay were equivalent to those of the reference standards and the competitor's equivalent products.

Figure 3. Excellent Binding Specificity

Use BunnyQuant dsDNA HS Assay Kit to quantify dsDNA, RNA, and a mixture of DNA and RNA (1:1). The results show that the product has high binding specificity for dsDNA and not for RNA.

Table 1. Excellent Tolerability to conventional contaminants

Using BunnyQuant dsDNA HS Assay Kit to quantify dsDNA from the samples containing conventional contaminants (e.g., salts, free nucleotides, proteins, solvents, etc.)，the results display that our product has excellent tolerability.

Figure 4. Fast dye binding speed

For 3 dsDNA samples with different concentrations, use BunnyQuant dsDNA HS Assay Kit to test every 1 min after the 1st min of addition, and record the change in concentration of the samples in 5 min. The product binds dsDNA quickly and reaches saturation within 1 min.

Figure 5-6. The DNA quantification results of different samples are in high agreement with competitive products.

Using the BunnyQuant dsDNA HS Assay Kit and ThermoFisher equivalent for quantification of DNA from different sample types and using a Qubit fluorometer and Elisa reader, respectively, the figure displays that our product is highly consistent with similar products from competing companies.

Product Component

Main Composition: Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Storage Conditions

The dsDNA reagent (stored away from light) and standards are stored at -20 ℃, and the 20X buffer is stored at 2-8 ℃. The kit is valid for 1 year.

Simple Workflow